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Please use this identifier to cite or link to this item:
http://hdl.handle.net/10174/7228
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Title: | Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building |
Authors: | David, Susana Sutre, AF Sanca, A Mané, A Henriques, V Portugal, Clara Sancho, Luísa Cardoso, A Paixão, Eleonora Duarte, EL Leite, CQF Salem, JI Antunes, Abílio |
Keywords: | Mycobacterium tuberculosis rpoB sputum smear microscopy sodium hypochlorite DHPLC sequencing |
Issue Date: | 2012 |
Publisher: | The Union |
Citation: | David, Susana; Sutre AF; Sanca A; Mané A; Henriques V; Portugal C; Sancho L; Cardoso A; Paixão, Eleonora; Duarte, EL; Leite CQF; Salem JI; Antunes A. Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building, International Journal of Tuberculosis and Lung Disease , 16, 11, 1535-1537, 2012. |
Abstract: | The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis
culture. Within a laboratory twinning arrangement,heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge,
this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings. |
URI: | http://hdl.handle.net/10174/7228 |
Type: | article |
Appears in Collections: | MVT - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica MED - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
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