Please use this identifier to cite or link to this item: http://hdl.handle.net/10174/7228

Title: Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
Authors: David, Susana
Sutre, AF
Sanca, A
Mané, A
Henriques, V
Portugal, Clara
Sancho, Luísa
Cardoso, A
Paixão, Eleonora
Duarte, EL
Leite, CQF
Salem, JI
Antunes, Abílio
Keywords: Mycobacterium tuberculosis
rpoB
sputum smear microscopy
sodium hypochlorite
DHPLC sequencing
Issue Date: 2012
Publisher: The Union
Citation: David, Susana; Sutre AF; Sanca A; Mané A; Henriques V; Portugal C; Sancho L; Cardoso A; Paixão, Eleonora; Duarte, EL; Leite CQF; Salem JI; Antunes A. Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building, International Journal of Tuberculosis and Lung Disease , 16, 11, 1535-1537, 2012.
Abstract: The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement,heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.
URI: http://hdl.handle.net/10174/7228
Type: article
Appears in Collections:MVT - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
MED - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

Files in This Item:

File Description SizeFormat
Bleach processingIJTLD0658-2012First page.pdf44.88 kBAdobe PDFView/Open
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpaceOrkut
Formato BibTex mendeley Endnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Dspace Dspace
DSpace Software, version 1.6.2 Copyright © 2002-2008 MIT and Hewlett-Packard - Feedback
UEvora B-On Curriculum DeGois