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http://hdl.handle.net/10174/5896
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Title: | Adsorption of Bovine Serum Albumin onto Mesocellular Silica Foams with Differently Sized Cells and Windows |
Authors: | Sezões, F Ribeiro Carrott, MML Mourão, PAM Carrott, PJM |
Keywords: | Chemistry |
Issue Date: | 2010 |
Publisher: | Multiscience |
Citation: | Adsorption of Bovine Serum Albumin onto Mesocellular Silica Foams with
Differently Sized Cells and Windows, F. Sezões, M.M.L. Ribeiro Carrott, P.A.M. Mourão & P.J.M. Carrott, Adsorption Science & Technology, 28 (2010) 777-788 |
Abstract: | As a model protein with quite large dimensions, Bovine Serum
Albumin (BSA) has been used to evaluate the influence of the distinct pore
structural characteristics of three mesocellular foam (MCF) materials prepared
with or without the addition of ammonium fluoride and with varying
1,3,5-trimethylbenzene/Pluronic P123 (TMB/P123) ratios. SBA-15 was also
studied for comparative purposes. Characterisation by X-ray diffraction and
electron microscopy confirmed the characteristic spheroid cell structure of the
MCF pores. Nitrogen adsorption/desorption isotherms at 77 K revealed the
different pore structural parameters of the MCF, viz. pore volume (1.8–2.4 cm3/g),
cell size (24.6–28.5 nm) and window size (11.3–17.3 nm), as obtained by the
NLDFT method. The equilibrium adsorption isotherms and the kinetic
adsorption data for BSA at 298 K and pH 5 were well fitted by the Langmuir
model and pseudo-second-order kinetic model, respectively.
The results showed that adsorption onto the material possessing a window size
of 11.3 nm was mostly restricted to the external surface, while a considerable
increase in the maximum adsorption capacity from 120 mg/g to 500–600 mg/g
was observed when the window size was above the critical dimension of 13.9 nm.
In the latter cases, the maximum adsorption capacities could be related to the pore
volume rather than to the total surface area. The confined BSA molecules were
strongly immobilised in the cells, since only a small proportion was desorbed
from the material with windows of 17.3 nm dimensions on contact with a buffer
solution of pH 7. |
URI: | http://www.multi-science.co.uk/adsorpt.htm http://hdl.handle.net/10174/5896 |
Type: | article |
Appears in Collections: | CQE - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
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