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Title: Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation
Authors: Branco, Sandra
Orvalho, João
Leitão, Alexandre
Pereira, Isadora
Malta, Manuel
Mariano, Isabel
Carvalho, Tânia
Baptista, Rui
Shiels, Brian R.
Peleteiro, Maria C.
Editors: Lee, Heungshik S.
Keywords: lymphoid cell proliferation
tropical theileriosis
Issue Date: Mar-2010
Publisher: Journal of Veterinary Science 11 (1), 27-34
Citation: Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation. Sandra Branco, João Orvalho, Alexandre Leitão, Isadora Pereira, Manuel Malta, Isabel Mariano, Tânia Carvalho, Rui Baptista, Brian R. Shiels, Maria C. Peleteiro (2010). Journal of Veterinary Science 11 (1), 27-34
Abstract: The authors describe 15 cases of acute lethal infection of calves (≤ 4 months of age) by the protozoan parasite Theileria annulata in the south of Portugal. Calves developed multifocal to coalescent nodular skin lesions, similar to multicentric malignant lymphoma. Infestation with ticks (genus Hyalomma) was intense. Theileria was seen in blood and lymph node smears, and Theileria annulata infection was confirmed by isolation of schizont-transformed cells and sequencing of hypervariable region 4 of the 18S rRNA gene. At necropsy, hemorrhagic nodules or nodules with a hemorrhagic halo were seen, particularly in the skin, subcutaneous tissue, skeletal and cardiac muscle, pharynx, trachea and intestinal serosa. Histologically nodules were formed by large, round, lymphoblastoid neoplastic-like cells. Immunohistochemistry (IHC) identified these cells as mostly CD3 positive T lymphocytes and MAC387 positive macrophages. A marker for B lymphocytes (CD79αcy) labelled very few cells. Theileria annulata infected cells in these nodules were also identified by IHC, through the use of two monoclonal antibodies (1C7 and 1C12) diagnostic for the parasite. It was concluded that the pathological changes observed in the different organs and tissues were caused by proliferation of schizont-infected macrophages, which subsequently stimulate a severe uncontrolled proliferation of uninfected T lymphocytes.
Type: article
Appears in Collections:MED - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

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