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|Title: ||cEpiderm, a Canine Skin Analog Suitable for In Vivo Testing Replacement|
|Authors: ||Marques, Mariana|
|Keywords: ||histotypical skin culture|
|Issue Date: ||20-Oct-2022|
|Citation: ||Marques, M.; Nunes, J.;
Ustymenko, B.; Fialho, L.; Martins, L.;
Burke, A.J.; Filho, C.; Craveiro, A.C.;
Costa, A.R.; Branco, S.; et al.
cEpiderm, a Canine Skin Analog
Suitable for In Vivo Testing
Replacement. BioChem 2022, 2,
|Abstract: ||Skin is one of the organs most tested for toxicity and safety evaluation during the process
of drug research and development and in the past has usually been performed in vivo using animals.
Over the last few years, non-animal alternatives have been developed and validated epidermis
models for human and rat skin are already available. Our goal was to develop a histotypical
canine skin analog, suitable for non-animal biocompatibility and biosafety assessment. Canine
keratinocytes were seeded in an air-lift culture using an adapted version of the CELLnTEC protocol.
Corrosion and irritation protocols were adapted from human EpiSkinTM. For histological analysis,
sample biopsies were fixed in neutral-buffered formalin, and paraffin slices were routinely processed
and stained with hematoxylin and eosin. A canine multilayer and stratified epidermal-like tissue
(cEpiderm), confirmed by histological analysis, was obtained. The cEpiderm tissue exhibited normal
morphological and functional characteristics of epidermis, namely impermeability and an adequate
response to stressors. The cEpiderm is a promising canine skin model for non-animal safety testing
of veterinary pharmaceuticals and/or cosmetics, significantly contributing to reducing undesirable
in vivo approaches. cEpiderm is therefore a valid canine skin model and may be made commercially
available either as a service or as a product.|
|Appears in Collections:||ICT - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica|
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