Please use this identifier to cite or link to this item:

Title: Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region
Authors: Campos, Maria Doroteia
Varanda, Carla
Materatski, Patrick
Félix, Maria Rosário
Keywords: grapevine trunk disease
Vivis vinifera
fungal genetic classification
Issue Date: 2019
Citation: de Almeida, A.B.; Concas, J.; Campos, M.D. , Varanda, C.M.R.; Materatski, P.; Murolo, S.; Romanazzi, G.; Félix, M.R. Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region. (2019). I Jornadas MED (Mediterranean Institute for Agriculture, Environment and Development). 27-28 June, Évora, Portugal, Book of abstracts, p. 117.
Abstract: Grapevine trunk diseases (GTDs) are one of the most important fungal diseases affecting grapevine plants in all the major growing regions of the world (Pintos et al, 2018)1 with up to 133 fungal species belonging to 34 genera associated to this disease (Gemaje et al, 2018)2. Management of GTDs is difficult and its complete eradication is not possible. For these reasons, its control is meanly focused on disease prevention and mitigation (U´rbez-Torres 2011)3.The work here presented had the aim to identify molecularly the endophytic fungi present in different tissues of grapevines, and among them the fungi responsible for GTDs. Pure fungal cultures were isolated from diseased grapevines plants belonging to two cultivars of Alentejo region (‘Trincadeira’ and ‘Alicante Bouchet’). Mycelium was then harvested, gDNA was extracted (Doyle and Doyle, 1987), and for fungal genetic classification the ribosomal internal transcribed spacer (ITS) region of nuclear rDNA was amplified through PCR by using ITS1 and ITS4 primers (White et al. 1990)4. All the fungi sequences that showed resemblance of 98% were used to classify the fungus analysed. The most prevalent grapevine trunk disease fungi identified were Hormonema viticola, Stereum armeniaccum, Philophorafastigiata, Diaporthe, Truncatella angustata, Cytospora acaciae, Diplodia pseudoseriata, Neofusicocum parvum, and Pestalotiopsis sp.
Type: lecture
Appears in Collections:MED - Comunicações - Em Congressos Científicos Nacionais

Files in This Item:

File Description SizeFormat
Abstract MED_A.B. de Almeida.pdf319.42 kBAdobe PDFView/Open
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpaceOrkut
Formato BibTex mendeley Endnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


Dspace Dspace
DSpace Software, version 1.6.2 Copyright © 2002-2008 MIT and Hewlett-Packard - Feedback
UEvora B-On Curriculum DeGois