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Title: Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies
Authors: Lozano-Ojalvo, Daniel
Benedé, Sara
M. Antunes, Célia
L. Bavaro, Simona
Bouchaud, Grégory
Costa, Ana
Denery-Papini, Sandra
Díaz-Perales, Araceli
Garrido-Arandia, María
Gavrovic-Jankulovic, Marija
Hayen, Simone
Wróblewska, Mónica
E.M. Willemsen, Linette
L. Roggen, Erwin
H.M. van Bilsen, Jolanda
Keywords: AOP
in vitro
Issue Date: 2019
Citation: Daniel Lozano-Ojalvo, Sara Benedé, Celia M. Antunes, Simona L. Bavaro, Grégory Bouchaud, Ana Costa, Sandra Denery-Papini, Araceli Díaz-Perales, María Garrido-Arandia, Marija Gavrovic-Jankulovic, Simone Hayen, Mónica Martínez-Blanco, Elena Molina, Linda Monaci, Raymond H.H. Pieters, Clelia Villemin, Harry J. Wichers, Barbara Wróblewska, Linette E.M. Willemsen, Erwin L. Roggen, Jolanda H.M. van Bilsen, Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies, Trends in Food Science & Technology, Volume 85, 2019, Pages 307-319. ISSN 0924-2244.
Abstract: Background Before introducing proteins from new or alternative dietary sources into the market, a compressive risk assessment including food allergic sensitization should be carried out in order to ensure their safety. We have recently proposed the adverse outcome pathway (AOP) concept to structure the current mechanistic understanding of the molecular and cellular pathways evidenced to drive IgE-mediated food allergies. This AOP framework offers the biological context to collect and structure existing in vitro methods and to identify missing assays to evaluate sensitizing potential of food proteins. Scope and approach In this review, we provide a state-of-the-art overview of available in vitro approaches for assessing the sensitizing potential of food proteins, including their strengths and limitations. These approaches are structured by their potential to evaluate the molecular initiating and key events driving food sensitization. Key findings and conclusions The application of the AOP framework offers the opportunity to anchor existing testing methods to specific building blocks of the AOP for food sensitization. In general, in vitro methods evaluating mechanisms involved in the innate immune response are easier to address than assays addressing the adaptive immune response due to the low precursor frequency of allergen-specific T and B cells. Novel ex vivo culture strategies may have the potential to become useful tools for investigating the sensitizing potential of food proteins. When applied in the context of an integrated testing strategy, the described approaches may reduce, if not replace, current animal testing approaches.
Type: article
Appears in Collections:QUI - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
ICT - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

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