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|Title: ||detection for yeast and bacteria in wood slabs by RNA FISH|
|Authors: ||Vieira, Ricardo|
González Pérez, Marina
Caldeira, Ana Teresa
|Keywords: ||Cultural Heritage|
|Issue Date: ||Jul-2018|
|Citation: ||Vieira, R.; González-Pérez, M.; Candeias, A.; Caldeira, A. T. Detection for yeast and bacteria in wood slabs by RNA-FISH. Ciência 2018, Encontro com a Ciência e Tecnologia em Portugal. 2018, July, 2, Lisboa (Portugal).|
|Abstract: ||The deterioration of cultural heritage objects and assets (mural paintings, statues, and many other art objects made of wood, stone, paper, ceramic, glass, inter alia) can be
caused by microorganisms . One of the most important steps for applying adequate conservation and protection measures is early identification and monitorization of
microbial colonization. The conventional culture-based methods used so far have become insufficient to detect/identify the biodeteriogenic agents. Thus, molecular techniques
have started to attract considerable interest [2,3].
Our group is focused on detecting and identifying microorganisms that cause biodeterioration on artworks using the RNA-FISH molecular technique . It is a simple, rapid and
promising molecular technique enabling the detection, visualization and identification of the viable microorganisms of interest [5,6].
As any other technique, RNA-FISH has its own minimum Limit Of Detection (LOD) and for ensuring the reliability of RNA-FISH analyses, determination of the associated LODs
is imperative. Thus, the aim of this work was to determine the LOD for yeast and bacteria in wood slabs by RNA-FISH. Universal probes for targeting eukaryotes (EUK516) and
bacteria (EUB338) labeled with Cy3 or Atto-647N dyes were used.|
|Appears in Collections:||HERCULES - Comunicações - Em Congressos Científicos Nacionais|
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