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Title: detection for yeast and bacteria in wood slabs by RNA FISH
Authors: Vieira, Ricardo
Candeias, António
González Pérez, Marina
Caldeira, Ana Teresa
Caldeira, A
Keywords: Cultural Heritage
Issue Date: Jul-2018
Publisher: FCT
Citation: Vieira, R.; González-Pérez, M.; Candeias, A.; Caldeira, A. T. Detection for yeast and bacteria in wood slabs by RNA-FISH. Ciência 2018, Encontro com a Ciência e Tecnologia em Portugal. 2018, July, 2, Lisboa (Portugal).
Abstract: The deterioration of cultural heritage objects and assets (mural paintings, statues, and many other art objects made of wood, stone, paper, ceramic, glass, inter alia) can be caused by microorganisms [1]. One of the most important steps for applying adequate conservation and protection measures is early identification and monitorization of microbial colonization. The conventional culture-based methods used so far have become insufficient to detect/identify the biodeteriogenic agents. Thus, molecular techniques have started to attract considerable interest [2,3]. Our group is focused on detecting and identifying microorganisms that cause biodeterioration on artworks using the RNA-FISH molecular technique [4]. It is a simple, rapid and promising molecular technique enabling the detection, visualization and identification of the viable microorganisms of interest [5,6]. As any other technique, RNA-FISH has its own minimum Limit Of Detection (LOD) and for ensuring the reliability of RNA-FISH analyses, determination of the associated LODs is imperative. Thus, the aim of this work was to determine the LOD for yeast and bacteria in wood slabs by RNA-FISH. Universal probes for targeting eukaryotes (EUK516) and bacteria (EUB338) labeled with Cy3 or Atto-647N dyes were used.
Type: lecture
Appears in Collections:HERCULES - Comunicações - Em Congressos Científicos Nacionais

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