Please use this identifier to cite or link to this item:
http://hdl.handle.net/10174/19541
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| Title: | Fixing, storage time and DNA extraction applied to dT-RFLP for ecological and molecular studies of marine nematodes assemblages |
| Authors: | Avó, Ana patrícia
Mitchell, Susan
Daniell, Tim
Nielson, Roy
Adão, Helena |
| Keywords: | Fixing
Storage
DNA
barcoding |
| Issue Date: | 4-Jul-2016 |
| Publisher: | ISIMCO Conference- 16th International meiofauna conference |
| Citation: | Avó A.P., Susan Mitchell, Tim Daniell, Roy Nielson, H. Adão (2016). Fixing, storage time and DNA extraction applied to DT-RFLP for ecological and molecular studies of marine nematode assemblages, IMCO Conference- 16th International meiofauna conference, 3-8 July, Creta, Greece, p. 131-132 |
| Abstract: | The application of molecular methods offers an alternative faster than traditional methods based on morphology
It is nearly impossible to process all the samples in short period using traditional methods, and the deterioration of marine sediments rapidly occurs
The dT-RFLP (directed Terminal-Restriction Fragment Length Polymorphism) allows a rapid assessment of biodiversity changes of nematodes assemblages
The use of a not suitable fixing, storage time and DNA extraction could be a limitation in molecular analysis like dT-RFLP and real time PCR.Objetives: the best fixative
•the level of DNA degradation over the time
•the best DNA extraction method
for marine nematodes and suitable for dT-RFLP analysis |
| URI: | http://hdl.handle.net/10174/19541 |
| Type: | lecture |
| Appears in Collections: | BIO - Comunicações - Em Congressos Científicos Internacionais
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