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Title: RNA-FISH Technique: Probe Cocktails vs Single Probes
Authors: Vieira, Ricardo
Pereira, António
Candeias, António
Caldeira, A. Teresa
González-Pérez, Marina
Keywords: Fluorescence In Situ Hybridization
Probe cocktails
Rhodotorula sp
Issue Date: Nov-2016
Publisher: SPB2016- XIX National Congress of Biochemistry
Citation: Ricardo Vieira, António Pereira, António Candeias, Ana T. Caldeira, Marina González-Pérez. RNA-FISH: Probe Cocktails vs Single Probes. SPB2016 - Book of Abstracts XIX National Congress of Biochemistry. No. P4/11, Guimarães, Portugal, Dec 8-10, 84, 2016.
Abstract: RNA-Fluorescence In Situ Hybridization (RNA-FISH) enables to analyze and visualize the microorganisms of interest within microbial communities in their natural environments by fluorescent labelling of specific RNA sequences. Poor accessibility or low content of the RNA target region can cause false positives/negatives due to low fluorescence of the cells. To reduce the chances of this occurring, probe cocktails – i.e. mixture of several probes that hybridize to different regions of the target RNA- has been proposed as an alternative to single probes use for increasing the Fluorescence Intensities (FI). However, is this really a good solution? The key finding of this work was that the use of probe cocktails is not always a good solution for maximizing the FI as at high concentrations the single probe EUK516-6 FAM yielded higher FI than the probe cocktails.
Type: lecture
Appears in Collections:HERCULES - Comunicações - Em Congressos Científicos Nacionais

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