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Title: Performance of an indigenous b-mercaptoethanolmodified antigen in comparison with a commercial reference in direct agglutination test for detection of canine visceral leishmaniasis
Authors: Semião-Santos, Saul
Barral Veloso, Laura
Paes de Andrade, Paulo
Almeida de Melo, Márcia
Martins, Luís
Marinho, Artur
Afonso de Almeida, José
Campino, Lénea
Harith, Abdallah
Editors: Borriello, S. Peter
Keywords: Leishmania
Issue Date: Jan-2014
Publisher: Society for General Microbiology (SGM)
Citation: J Med Microbiol. 2014. 63:106-10.
Abstract: We compared the performance of a locally produced b-mercaptoethanol-modified promastigote antigen (b-ME-Ag) of an indigenous Leishmania infantum strain against that of a trypsinized Leishmania donovani reference (REF-Ag) in the direct agglutination test (DAT) for detection of canine visceral leishmaniasis (CVL). One hundred and fifty-one serum samples collected from dogs belonging to four groups with different conditions were included. At a DAT titre of 1 : 320, statistically determined as optimal cut-off value for b-ME-Ag, and 1 : 160 for REF-Ag, a sensitivity and a specificity of 100% were estimated for b-ME-Ag in comparison with 96.6% and 100 %, respectively, for REF-Ag. Overall, levels of agglutination titres recorded for the two antigens were highly concordant (Cohen’s k50.879) in both the CVL and non-CVL groups. Based on current results, and ease experienced in processing the antigen and reading the test outcome, we recommend incorporation of b-ME-Ag in DAT for confirmation or exclusion of suspected CVL in dogs.
Type: article
Appears in Collections:MED - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

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