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Title: Olfactory transduction pathways in the Senegalese sole Solea senegalensis
Authors: Velez, Zélia
Hubbard, Peter C.
Barata, Eduardo N.
Canário, Adelino V.M.
Keywords: olfaction
Senegal sole
transduction mechanisms
Issue Date: 2013
Publisher: Wiley
Citation: Velez, Z., Hubbard, P. C., Barata, E. N. and Canário, A. V. M. (2013), Olfactory transduction pathways in the Senegalese sole Solea senegalensis. Journal of Fish Biology, 83: 501–514. doi: 10.1111/jfb.12185
Abstract: This study tested whether differences in sensitivity between the upper and lower olfactory epithelia of Solea senegalensis are associated with different odorant receptors and transduction pathways, using the electro-olfactogram. Receptor mechanisms were assessed by cross-adaptation with amino acids (L-cysteine, L-phenylalanine and 1-methyl-L-tryptophan) and bile acids (taurocholic acid and cholic acid). This suggested that relatively specific receptors exist for 1-methyl-L-tryptophan and L-phenylalanine (food-related odorants) in the lower epithelium, and for taurocholic acid (conspecific-derived odorant) in the upper. Inhibition by U73122 [a phospholipase C (PLC) inhibitor] suggested that olfactory responses to amino acids were mediated mostly, but not entirely, by PLC-mediated transduction (IC50; 15–55 nM), whereas bile acid responses were mediated by both PLC and adenylate cyclase–cyclic adenosine monophosphate (AC–cAMP) (using SQ-22536; an AC inhibitor). Simultaneous application of both drugs rarely inhibited responses completely, suggesting possible involvement of non-PLC and non-AC mediated mechanisms. For aromatic amino acids and bile acids, there were differences in the contribution of each transduction pathway (PLC, AC and non-PLC and non-AC) between the two epithelia. These results suggest that differences in sensitivity of the two epithelia are associated with differences in odorant receptors and transduction mechanisms.
Type: article
Appears in Collections:BIO - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

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