Please use this identifier to cite or link to this item:

Title: In Vitro Somatic Embryogenesis from Stamens of ‘Trincadeira’, a Portuguese Vitis vinifera L. Cultivar
Authors: Cardoso, Hélia
Peixe, A.
Pais, M.S.
Editors: Romano, A.
Keywords: microspore developmental stage
somatic embryos
ploidy levels
Issue Date: 2009
Publisher: ISHS
Citation: Cardoso, H.G., Peixe, A.V. and Pais, M.S. (2009). In vitro somatic embryogenesis from stamens of 'Trincadeira' a portuguese Vitis vinifera l. Cultivar. Acta Hort., 812:305-312
Abstract: Stamens collected from vineyard-growing plants were used as starting material to induce in vitro somatic embryogenesis in ‘Trincadeira’, a Portuguese grapevine Vitis vinifera cultivar. Stamens filaments bearing anthers enclosing microspores in the binucleate stage were the most responsive for embryogenesis induction. Culture media with low concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (5 µM) and 6- benzylaminopurine (BAP) (1 µM), presented the highest efficiency for embryogenic calluses formation. Calluses, formed at the stamen excision zone, with white to paleyellow colour and granular appearance, revealed embryogenic competence. Embryo formation was achieved in Nitsch and Nitsch (1969) basal medium supplemented with 1 or 5 µM α-naphtaleneacetic acid (NAA). Embryos were converted into plants true to type that were successfully acclimatized and field planted. Plant ploidy, evaluated by flow cytometry, revealed that all the plants tested presented 2n=2x=38 chromosomes.
Type: article
Appears in Collections:MED - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
FIT - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

Files in This Item:

File Description SizeFormat
In Vitro Somatic Embryogenesis from Stamens of ‘Trincadeira’ pag1.pdf1.04 MBAdobe PDFView/OpenRestrict Access. You can Request a copy!
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpaceOrkut
Formato BibTex mendeley Endnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


Dspace Dspace
DSpace Software, version 1.6.2 Copyright © 2002-2008 MIT and Hewlett-Packard - Feedback
UEvora B-On Curriculum DeGois