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http://hdl.handle.net/10174/2886
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Title: | Effect of Acute Copper Sulfate Exposure on Olfactory Responses to Amino Acids and Pheromones in Goldfish (Carassius auratus) |
Authors: | K O L M A K O V, N I K O L A Y N H U B B A R D, P E T E R C L O P E S, O R L A N D O C A N A R I O, A D E L I N O V . M |
Keywords: | Electro-olfactogram recording Olfactory epithelium Scanning electron microscopy Pheromones Amino acids Goldfish |
Issue Date: | 2009 |
Abstract: | Exposure of olfactory epithelium to environmentally relevant
concentrations of copper disrupts olfaction in fish. To examine
the dynamics of recovery at both functional and morphological
levels after acute copper exposure, unilateral exposure of
goldfish olfactory epithelia to 100 μM CuSO4 (10 min) was
followed by electro-olfactogram (EOG) recording and scanning
electron microscopy. Sensitivity to amino acids (L-arginine
and L-serine), generally considered food-related odorants,
recovered most rapidly (three days), followed by that to
catecholamines(3-O-methoxytyramine),bileacids(taurolithocholic
acid) and the steroid pheromone, 17,20 -dihydroxy-4-pregnen-
3-one 20-sulfate, which took 28 days to reach full recovery.
Sensitivity to the postovulatory pheromone prostaglandin F2R had
not fully recovered even at 28 days. These changes in
sensitivity were correlated with changes in the recovery of
ciliated and microvillous receptor cell types. Microvillous cells
appeared largely unaffected by CuSO4 treatment. Cilia in
ciliated receptor neurones, however, appeared damaged one
day post-treatment and were virtually absent after three days but
had begun to recover after 14 days. Together, these results
support the hypothesis that microvillous receptor neurones detect
amino acids whereas ciliated receptor neurones were not
functional and are responsible for detection of social stimuli
(bile acidsandpheromones).Furthermore, differences in sensitivity
to copper may be due to different transduction pathways in
the different cell types. |
URI: | http://hdl.handle.net/10174/2886 |
Type: | article |
Appears in Collections: | BIO - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
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