Please use this identifier to cite or link to this item: http://hdl.handle.net/10174/2816

Title: Specific amino acids of Olive mild mosaic virus coat protein are involved in transmission by Olpidium brassicae
Authors: Varanda, Carla
Félix, Maria R.
Soares, Cláudio M.
Oliveira, Solange
Clara, M. Ivone
Keywords: TOBACCO NECROSIS VIRUS
PLANT-VIRUSES
Issue Date: 2011
Publisher: SOC GENERAL MICROBIOLOGY
Citation: Varanda, C.M., Félix, M.R., Soares, C.M., Oliveira, S., Clara, M.I. (2011) “Specific amino acids of Olive mild mosaic virus coat protein are involved on transmission by Olpidium brassicae” Journal of General Virology 92:2209 – 2213
Abstract: Abstract: Transmission of Olive mild mosaic virus (OMMV) is facilitated by Olpidium brassicae (Wor.) Dang. An OMMV mutant (OMMVL11) containing two changes in the coat protein (CP), asparagine to tyrosine at position 189 and alanine to threonine at position 216, has been shown not to be Olpidium brassicae-transmissible owing to inefficient attachment of virions to zoospores. In this study, these amino acid changes were separately introduced into the OMMV genome through site-directed mutagenesis, and the asparagine-to-tyrosine change was shown to be largely responsible for the loss of transmission. Analysis of the structure of OMMV CP by comparative modelling approaches showed that this change is located in the interior of the virus particle and the alanine-to-threonine change is exposed on the surface. The asparagine-to-tyrosine change may indirectly affect attachment via changes in the conformation of viral CP subunits, altering the receptor binding site and thus preventing binding to the fungal zoospore.
URI: http://hdl.handle.net/10174/2816
Type: article
Appears in Collections:ICAAM - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
BIO - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

Files in This Item:

File Description SizeFormat
Abstract.pdfAbstract83.05 kBAdobe PDFView/Open
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpaceOrkut
Formato BibTex mendeley Endnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Dspace Dspace
DSpace Software, version 1.6.2 Copyright © 2002-2008 MIT and Hewlett-Packard - Feedback
UEvora B-On Curriculum DeGois