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|Title: ||Strains of Mesorhizobium amorphae and M. tianshanense carrying symbiotic genes of common chickpea endosymbiotic species constitute a novel biovar (ciceri) able to nodulate Cicer arietinum|
|Authors: ||Rivas, Raul|
|Issue Date: ||2007|
|Abstract: ||Aims: To identify several strains of Mesorhizobium amorphae and Mesorhizobium
tianshanense nodulating Cicer arietinum in Spain and Portugal, and to
study the symbiotic genes carried by these strains.
Methods and Results: The sequences of 16S-23S intergenic spacer (ITS), 16S
rRNA gene and symbiotic genes nodC and nifH were analysed. According to
their 16S rRNA gene and ITS sequences, the strains from this study were identified
as M. amorphae and M. tianshanense. The type strains of these species
were isolated in China from Glycyrrhiza pallidiflora and Amorpha fruticosa nodules,
respectively, and are not capable of nodulating chickpea. These strains
carry symbiotic genes, phylogenetically divergent from those of the chickpea
isolates, whose nodC and nifH genes showed more than 99% similarity with
respect to those from Mesorhizobium ciceri and Mesorhizobium mediterraneum,
the two common chickpea nodulating species in Spain and Portugal.
Conclusions: The results from this study showed that different symbiotic genes
have been acquired by strains from the same species during their coevolution
with different legumes in distinct geographical locations.
Significance and Impact of the Study: A new infrasubspecific division named
biovar ciceri is proposed within M. amorphae and M. tianshanense to include
the strains able to effectively nodulate Cicer arietinum.|
|Appears in Collections:||MED - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica|
BIO - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
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