Please use this identifier to cite or link to this item:
|Title: ||Ectopic expression of Kip-related proteins restrains root-knot nematode-feeding site expansion|
|Authors: ||Vieira, Paulo|
Almeida Engler, Janice
|Keywords: ||Arabidopsis roots, cell cycle|
|Issue Date: ||Mar-2013|
|Publisher: ||New Phytologist Trust|
|Abstract: ||The development of nematode feeding sites induced by root-knot nematodes involves the
synchronized activation of cell cycle processes such as acytokinetic mitoses and DNA amplification.
A number of key cell cycle genes are reported to be critical for nematode feeding site
development. However, it remains unknown whether plant cyclin-dependent kinase (CDK)
inhibitors such as the Arabidopsis interactor/inhibitor of CDK (ICK)/Kip-related protein (KRP)
family are involved in nematode feeding site development. This study demonstrates the
involvement of Arabidopsis ICK2/KRP2 and ICK1/KRP1 in the control of mitosis to endoreduplication
in galls induced by the root-knot nematode Meloidogyne incognita.
! Using ICK/KRP promoter-GUS fusions and mRNA in situ hybridizations, we showed that
ICK2/KRP2, ICK3/KRP5 and ICK4/KRP6 are expressed in galls after nematode infection.
Loss-of-function mutants have minor effects on gall development and nematode reproduction.
Conversely, overexpression of both ICK1/KRP1 and ICK2/KRP2 impaired mitosis in giant
cells and blocked neighboring cell proliferation, resulting in a drastic reduction of gall size.
! Studying the dynamics of protein expression demonstrated that protein levels of ICK2/
KRP2 are tightly regulated during giant cell development and reliant on the presence of the
! This work demonstrates that impeding cell cycle progression by means of ICK1/KRP1 and
ICK2/KRP2 overexpression severely restricts gall development, leading to a marked limitation
of root-knot nematode development and reduced numbers of offspring|
|Appears in Collections:||BIO - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.