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Title: Cryopreservation of ovine in vitro produced embryos using centrifugation and cytocalasin D
Authors: Romão, Ricardo Jorge
Marques, Carla
Batista, Maria
Barbas, João
Horta, António
Carolino, Nuno
Bettencourt, Elisa
Pereira, Rosa
Editors: Willey, Blackwell
Keywords: embryo
cytocalasin D
Issue Date: Sep-2013
Publisher: Willey Blackwell
Citation: Romão, R; Marques, C; Batista, M; Barba, J; Horta, A; Carolino, N; Bettencourt, E, Pereira, R. (2013). Cryopreservation of ovine in vitro produced embryos using centrifugation and cytocalasin D. Proceedings of the 17th Annual Conference of the European Society for domestic animal reproduction (ESDAR). Bologna, Italy. 12-14th september. Reproduction in domestic animals, 48 (Supple.1) pp 104. DOI: 10.1111/rda.12228.
Abstract: The cryosurvival of ovine in vitro produced embryos are still low, thus preventing its routine transfer. Attempts have been made to override this problem by decreasing embryos lipid content or protecting their structure with cytoskeletal stabilizers. In this study we used embryo mechanical delipidation through centrifugation in the presence or absence of cytocalasin D testing its effect on embryo quality and cryosurvival. Mature ovine oocytes (n=1146) were inseminated using fresh semen of a Merino ram. After assessing cleavage, embryo development proceeded until the blastocyst stage. Prior to vitrification, embryos were randomly distributed to the following groups: control (n= 20), without treatment; centrifugation (n=18), blastocysts were centrifuged at 15000g; cytochalasin D (n=20), embryos were treated with 5 µg mL-1 cytocalasin D; centrifugation+cytocalasin D (n=17), embryos were treated with both centrifugation and cytocalasin D. Embryos integrity and re-expansion were assessed post-warming and after 3 hours of culture. Post-warming integrity rate was lowest (p=0.04) in embryos of centrifugation group. No differences were identified among groups for re-expansion rates. A possible role of cytocalasin D in protecting mechanical damage of centrifuged embryos during cryopreservation was identified. However this stabilizer alone did not improve the quality of warmed embryos when compared to control.
ISSN: 0936-6768
Type: article
Appears in Collections:MVT - Artigos em Livros de Actas/Proceedings

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