Please use this identifier to cite or link to this item: http://hdl.handle.net/10174/9778

Title: Performance of an indigenous β-mercaptoethanol-modified antigen in comparison with a commercial reference in direct agglutination test for detection of canine visceral leishmaniasis
Authors: Semião-Santos, Saul
Veloso, Laura
Andrade, Paulo
Melo, Márcia
Martins, Luís
Marinho, Artur
Almeida, José
Campino, Lénea
Harith, Abdallah
Keywords: leishmaniasis
agglutination test
b-mercaptoethanol- modified antigen
Issue Date: 18-Oct-2013
Publisher: J Med Microbiol January 2014 63:106-110
Citation: Semião- Santos, S., Veloso, L., Andrade, P., Melo, M., Martins, L., Marinh, A., Almeida, J., Campino, L. and Harith, A. (2013). “Performance of an indigenous β-mercaptoethanol-modified antigen in comparison with a commercial reference in direct agglutination test for detection of canine visceral leishmaniasis”, in J Med Microbiol, January 2014 63:106-110.
Abstract: We compared the performance of a locally produced b-mercaptoethanol-modified promastigote antigen (b-ME-Ag) of an indigenous Leishmania infantum strain against that of a trypsinized Leishmania donovani reference (REF-Ag) in the direct agglutination test (DAT) for detection of canine visceral leishmaniasis (CVL). One hundred and fifty-one serum samples collected from dogs belonging to four groups with different conditions were included. At a DAT titre of 1 : 320, statistically determined as optimal cut-off value for b-ME-Ag, and 1 : 160 for REF-Ag, a sensitivity and a specificity of 100 % were estimated for b-ME-Ag in comparison with 96.6 % and 100 %, respectively, for REF-Ag. Overall, levels of agglutination titres recorded for the two antigens were highly concordant (Cohen’s k50.879) in both the CVL and non-CVL groups. Based on current results, and ease experienced in processing the antigen and reading the test outcome, we recommend incorporation of b-ME-Ag in DAT for confirmation or exclusion of suspected CVL in dogs.
URI: http://hdl.handle.net/10174/9778
Type: article
Appears in Collections:MVT - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
ZOO - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
ICAAM - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

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