Please use this identifier to cite or link to this item: http://hdl.handle.net/10174/8786

Title: Molecular approach to characterize ectomycorrhizae fungi from Mediterranean pine stands in Portugal
Authors: Ragonezi, Carla
Caldeira, A. Teresa
Martins, M. Rosário
Salvador, Cátia
Santos-Silva, Celeste
Ganhão, Elsa
Klimaszewska, Krystyna
Zavattieri, Amely
Keywords: Pisolithus arhizus
Lactarius deliciosus
Pinus pinea
M13-PCR
ITS
Issue Date: 16-Aug-2013
Publisher: Brazilian journal of Microbiology
Citation: RAGONEZI, Carla et al. Molecular approach to characterize ectomycorrhizae fungi from Mediterranean pine stands in Portugal. Braz. J. Microbiol. [online]. ahead of print [cited 2013-09-18], pp. 0-0 . Available from: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013005000035&lng=en&nrm=iso>. Epub Aug 16, 2013. ISSN 1517-8382.
Abstract: Stone pine (Pinus pinea L.), like other conifers, forms ectomycorrhizas (ECM), which have beneficial impact on plant growth in natural environments and forest ecosystems. An in vitro co-culture of stone pine microshoots with pure mycelia of isolated ECM sporocarps was used to overcome the root growth cessation not only in vitro but also to improve root development during acclimation phase. Pisolithus arhizus (Scop.) Rauschert and Lactarius deliciosus (L. ex Fr.) S.F. Gray fungi, were collected, pure cultured and used in in vitro co-culture with stone pine microshoots. Samples of P. arhizus and L. deliciosus for the in vitro co-cultures were collected from the pine stands southwest Portugal. The in situ characterization was based on their morphotypes. To confirm the identity of the collected material, ITS amplification was applied using the pure cultures derived from the sporocarps. Additionally, a molecular profile using PCR based genomic fingerprinting comparison was executed with other genera of Basidiomycetes and Ascomycetes. Our results showed the effectiveness of the techniques used to amplify DNA polymorphic sequences, which enhances the ­characte­rization of the genetic profile of ECM fungi and also provides an option to verify the fungus identity at any stage of plant mycorrhization.
URI: http://hdl.handle.net/10174/8786
ISSN: 1517-8382
Type: article
Appears in Collections:ICAAM - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

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