Please use this identifier to cite or link to this item: http://hdl.handle.net/10174/7228

Title: Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
Authors: David, Susana
Sutre, AF
Sanca, A
Mané, A
Henriques, V
Portugal, Clara
Sancho, Luísa
Cardoso, A
Paixão, Eleonora
Duarte, EL
Leite, CQF
Salem, JI
Antunes, Abílio
Keywords: Mycobacterium tuberculosis
rpoB
sputum smear microscopy
sodium hypochlorite
DHPLC sequencing
Issue Date: 2012
Publisher: The Union
Citation: David, Susana; Sutre AF; Sanca A; Mané A; Henriques V; Portugal C; Sancho L; Cardoso A; Paixão, Eleonora; Duarte, EL; Leite CQF; Salem JI; Antunes A. Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building, International Journal of Tuberculosis and Lung Disease , 16, 11, 1535-1537, 2012.
Abstract: The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement,heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.
URI: http://hdl.handle.net/10174/7228
Type: article
Appears in Collections:MVT - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
ICAAM - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

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