Please use this identifier to cite or link to this item: http://hdl.handle.net/10174/6441

Title: Effects of carbon source, carbon concentration and culture conditions on in vitro rooting of Pinus pinea L. microshoots
Authors: Zavattieri, Maria Amely
Lima, Mónica
Sobral, Virginia
Oliveira, Paulo Guilherme de
Costa, Alexandra Rosa da
Editors: Romano, A.
Keywords: Stone pine
rhizogenesis
glucose
sucrose
temperature
light
acclimation
Issue Date: 28-Feb-2009
Publisher: ISHS
Citation: Zavattieri, A., Lima, M., Sobral, V., Oliveira, P. and Costa, A. 2009. EFFECTS OF CARBON SOURCE, CARBON CONCENTRATION AND CULTURE CONDITIONS ON IN VITRO ROOTING OF PINUS PINEA L. MICROSHOOTS . Acta Hort. (ISHS) 812:173-180 http://www.actahort.org/books/812/812_19.htm
Abstract: In stone pine (Pinus pinea L.), clonal propagation via adventitious shoot formation from cotyledons has been reported before but rooting of these shoots is poor. The number of rooted shoots had low frequency limiting the used of micropropagation protocols as a choice for mass propagation of superior genotypes. Therefore the main objective of the present work was to increase the number and quality of roots per shoot in order to ensure the survival and growth of a great number of plants in the acclimation phase. To achieve this, different combinations of carbon source (sucrose or glucose) at different concentrations, under different environmental conditions (temperature and light), were tested in the induction and expression phases of the adventitious root formation. Shoots of different clones obtained via organogenesis have been employed for the experiments. Observations were made on the rooting percentage, root length and number of roots per shoot. The results showed a general increased of the number of roots per shoot and an earlier root formation when glucose was used as a carbon source. However, there were no differences in the percentage of rooting between the carbons sources tested. The best results were obtained using 0.117 M of glucose and dark treatment combined with 19°C during the induction phase of the rhizogenic process. Light and low sugar concentration proved to be beneficial for the expression phase, increasing the root length. A remarkable interclonal difference in the ability to form roots was observed. It was possible to obtain a rooting percentage of more than 75% in several of the tested clones.
URI: http://hdl.handle.net/10174/6441
ISBN: 978-90-66050-87-7
ISSN: 0567-7572
Type: article
Appears in Collections:ICAAM - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

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