Please use this identifier to cite or link to this item:

Title: Proteomic study of proteolysis during ripening of cheese made with Cynara cardunculus L.
Authors: Garrido, Ana Lúcia
Freitas, Sofia
Silva, Flávio
Lamy, Elsa
Conceição, Cristina
Issue Date: 9-Dec-2020
Publisher: UE – Universidade de Évora
Citation: Garrido, A.L., Freitas, S., Silva, F., Lamy, E., Conceição, C. 2020. Proteomic study of proteolysis during ripening of cheese made with Cynara cardunculus L. V PhD Students Meeting in Environmental and Agriculture, a 9 de dezembro de 2020. Livro de resumos, pág. 19. ISBN: 978-972-778-179-9.
Abstract: The pistils of wild and cultivated cardoons are used as vegetal coagulant to produced several traditional ewe´s and goat´s cheeses, some of them benefit from protected designation of origin (PDO) status in which Cynara cardunculus flower extracts have been successfully employed and legally required. The type of coagulant agent is one of the main factors responsible for the variability in cheese characteristics, being therefore its effect on the proteolysis a subject of profound study. A precise analytical technique is essential to understand the impact of different factors on cheese proteolysis. In this study three electrophoretic techniques have been used to evaluated the cheese proteolysis namely (1) urea polyacrylamide gel electrophoresis (Urea-PAGE) and (2) sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) for the insoluble fraction of cheese and (3) twodimensional gel electrophoresis (2-DE) for the soluble fraction. Results showed that urea-PAGE was the best method for cheese insoluble fraction analysis. Both urea PAGE and 2-DE methods are complementary and proteomic tools are helpful in understanding the proteolysis of ewe’s cheese.
Type: lecture
Appears in Collections:MED - Comunicações - Em Congressos Científicos Nacionais

Files in This Item:

File Description SizeFormat
Garrido 2020 EEDAA.pdf397.34 kBAdobe PDFView/Open
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpaceOrkut
Formato BibTex mendeley Endnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


Dspace Dspace
DSpace Software, version 1.6.2 Copyright © 2002-2008 MIT and Hewlett-Packard - Feedback
UEvora B-On Curriculum DeGois