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|Title: ||The dual role of Plant Viruses in CRISPR|
|Authors: ||Varanda, Carla|
|Issue Date: ||2020|
|Citation: ||5) Varanda, C.M.R.; Félix, M.D.R.; Campos M.D.; Patanita, M.; Materatski, P. (2020). The dual role of Plant Viruses in CRISPR. Abstract book of the V PhD Students Meeting in Environmental and Agriculture, University of Évora. Évora (Portugal), 9 de dezembro.|
|Abstract: ||Plant viruses cause devastating diseases in many agriculture systems, being a serious threat for
the provision of adequate nourishment to a continuous growing population. At the present
there are no chemical products that directly target the viruses, and their control rely mainly on
preventive sanitary measures to reduce viral infections that, although important, have proved
to be far from enough. The current most effective and sustainable solution is the use of virusresistant
varieties, which require too much work and time to obtain. In the recent years, the
versatile gene editing technology known as CRISPR/Cas has simplified the engineering of crops
and has successfully been used for the development of viral resistant plants. CRISPR stands for
Clustered regularly interspaced short palindromic repeats and CRISPR-associated (Cas) proteins,
and is based on a natural adaptive immune system that most archaeal and some bacterial
species present to defend themselves against invading bacteriophages.
Plant viral resistance using CRISPR/Cas technology has been achieved either through
manipulation of plant genome (plant-mediated resistance), by mutating host factors required
for viral infection, or through manipulation of virus genome (virus-mediated resistance), for
which CRISPR/Cas systems must specifically target and cleave viral DNA or RNA.
Viruses present an efficient machinery and comprehensive genome structure and, in a different
perspective, they have been used as biotechnological tools in several areas such as medicine,
materials industry and agriculture with several purposes. Due to all this potential, it is not
surprising that viruses have also been used as vectors for CRISPR technology, namely to deliver
CRISPR components into plants, a crucial step for the success of CRISPR technology.
Here we discuss the basic principles of CRISPR/Cas technology, with a special focus on the
advances of CRISPR/Cas to engineer plant resistance against DNA and RNA viruses. We also
describe several strategies for the delivery of these systems into plant cells, focusing on the
advantages and disadvantages of the use of plant viruses as vectors. We conclude by discussing
the constrains faced by the application of CRISPR/Cas technology in agriculture and future
|Appears in Collections:||MED - Artigos em Livros de Actas/Proceedings|
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