Please use this identifier to cite or link to this item: http://hdl.handle.net/10174/23876

Title: Saccharomyces cerevisiae exposed to titanium dioxide nanoparticles of less than 100 nm in size under heat shock partially reverted glucose-mediated repression of the citrate cycle
Authors: Capela-Pires, J
Ferreira, R
Alves-Pereira, I
Editors: Méndez-Vilas, A.
Keywords: alkaline phosphatase
pyruvate kinase
citrate synthase
succinate dehydrogenase
nanomaterials
Issue Date: 2018
Publisher: Formatex, Research Center
Citation: Capela-Pires J, Ferreira R, Alves-Pereira I. (2018) Chapter title: "Saccharomyces cerevisiae exposed to titanium dioxide nanoparticles of less than 100 nm in size under heat shock partially reverted glucose-mediated repression of the citrate cycle" In: A. Méndez-Vilas (ed), Global progress in applied microbiology: a multidisciplinary approach. 1sd ed Formatex, Research Center, Badajpoz, Spain. pp: 79-83 (both included). ISBN-13: 978-84-947512-2-6
Abstract: Energy metabolism in yeast cells can be manipulated by providing different carbon sources: Saccharomyces cerevisiae grown on glucose rapidly proliferates by means of fermentation, whereas in non-fermentable carbon sources, such as glycerol, metabolism shifts towards respiration. In general, fermentation is preferred by the yeast in rapidly proliferating cells even in the presence of oxygen, a process also called the Crabtree effect. In the present study, it was observed that the addition of glucose to S. cerevisiae UE-ME3 grown in a glycerol-rich medium caused an increase in the growth-marker enzyme alkaline phosphatase and a blockage of respiratory enzymes. In addition, the simultaneous exposure of yeast cells to TiO2-NP <100 nm (5 μg/mL) under heat shock (28/40), after 100 min of culture in respiratory-fermentative mode, caused a partial reversion of glucose-mediated repression of citrate synthase and succinate dehydrogenase activities, key-enzymes of the citrate cycle, and a decrease in the level of alkaline phosphatase activity similar to those detected in cells grown in respiratory mode.
URI: http://hdl.handle.net/10174/23876
ISBN: 978-84-947512-2-6
Type: article
Appears in Collections:ICAAM - Artigos em Livros de Actas/Proceedings

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