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|Title: ||Challenges in the rabbit haemorrhagic disease 2 (RHDV2) molecular diagnosis of vaccinated rabbits|
|Authors: ||Carvalho, C.L.|
Duarte, Margarida Dias
|Issue Date: ||2017|
|Citation: ||Carvalho, C.L.; Duarte, E.L.; Monteiro, M.; Botelho, A; Albuquerque, T.; Fevereiro, M.; Henriques, A.M.; Barros, S.; Duarte, Margarida Dias. Challenges in the rabbit haemorrhagic disease 2 (RHDV2) molecular diagnosis of vaccinated rabbits, Veterinary Microbiology, 198, 43-50, 2017.|
|Abstract: ||Molecular methods are fundamental tools for the diagnosis of viral infections. While interpretation of
results is straightforward for unvaccinated animals, where positivity represents ongoing or past
infections, the presence of vaccine virus in the tissues of recently vaccinated animals may mislead
In this study, we investigated the interference of RHDV2 vaccination in the results of a RT-qPCR for
RHDV2 detection, and possible associations between mean Cq values of
five animal groups differing in
age, vaccination status and origin (domestic/wild).
Viral sequences from vaccinated rabbits that died of RHDV2 infection (n = 14) were compared with the
sequences from the commercial vaccines used in those animals. Group Cq means were compared through
Independent t-test and One-way ANOVA.
We proved that RHDV2 vaccine-RNA is not detected by the RT-qPCR as early as 15 days post-
vaccination, an important fact in assisting results interpretation for diagnosis.
Cq values of vaccinated and non-vaccinated infected domestic adults showed a statistically significant
0.05), demonstrating that vaccination-induced immunity reduces viral loads and delays
disease progression. Contrarily, in vaccinated young rabbits higher viral loads were registered compared
to non-vaccinated kittens. No significant variation (p = 0.3824) was observed between viral loads of non-
vaccinated domestic and wild RHDV2-victimised rabbits. Although the reduced number of vaccinated
young animals analysed hampered a robust statistical analysis, this occurrence suggests that passively
acquired maternal antibodies may inhibit the active immune response to vaccination, delaying
protection and favouring disease progression.
finding emphasises the importance of adapting kitten RHDV2 vaccination schedules to circumvent
this interference phenomenon.|
|Other Identifiers: ||03781135|
|Appears in Collections:||MVT - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica|
MED - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
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