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Title: Establishment of antibody panels and histochemical techniques in routine tumor diagnosis in Veterinary Pathology
Authors: Pires, MA
Gama, A
Pires, I
Seixas, F
Prada, J
Pinto, ML
Branco, S
Vala, H
Delgado, L
Faísca, P
Carvalho, I
Lima, C
Ochoa, C
Orge, L
Monteiro, M
Amorim, I
Gartner, F
Peleteiro, C
Carvalho, S
Gil da Costa, R
Alves, A
Keywords: antibody
veterinary pathology
Issue Date: May-2016
Publisher: Sociedade Portuguesa de Patologia Veterinária
Citation: Establishment of antibody panels and histochemical techniques in routine tumor diagnosis in Veterinary Pathology. Pires M.A; Gama A; Pires I.; Seixas F.; Prada J.; Pinto M.L.; Branco S.; Vala H.; Delgado L.; Faísca P.; Carvalho. I; Lima C.; Ochoa C.; Orge L.; Monteiro, M.; Amorim I.; Gartner F.; Peleteiro C.; Carvalho S.; Gil da Costa R.; Alves A. XXI Encontro da Sociedade Portuguesa de Patologia Animal – Patologia Veterinária: Para além do diagnóstico de rotina. (20 e 21 de maio de 2016, Instituto de Medicina Molecular, Lisboa, Portugal).Book of abstracts (pág 31-32)
Abstract: In Portugal, Veterinary Pathology is developing rapidly, and in recent years we assist to the emergence of private laboratories and the restructuring of universities,polytechnics and public laboratories.The Portuguese Society of Animal Pathology,through its actions and its associates has been keeping the discussion among its peers in order to standardizethe criteria of description,classification and evaluation of cases which are the subject of our daily work.One of the last challenges is associated with the use of routine histochemical techniques and immunohistochemistry, in an effort to establish standardized panels for tumour diagnosis, which could eventually reduce each analysis cost.For this purpose a simple survey was built, in which all collaborators answered questions about the markers used for carcinoma, sarcoma and round cell tumour diagnosis, as well as general questions related with the subject. We obtained twenty-one answered to the questions, from public and private laboratories.In general, in most cases immunohistochemical and histochemical methods are used for diagnosis.The wide spectrum cytokeratins are universally used to confirm carcinoma, and vimentin for sarcoma. The CD3 marker is used by all laboratories to identify T lymphocytes. For the diagnosis of B-cell lymphoma, the marker used is not consensual. In each laboratory there are different markers for more specific situations and only two labs perform PCR techniques for diagnosis. These data will be presented to promote extended discussion,namely to reach a consensus when different markers are used.
Type: article
Appears in Collections:MED - Artigos em Livros de Actas/Proceedings

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