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Title: Novos desenvolvimentos para a deteção de leveduras e bactérias presentes em argamassas
Other Titles: Development of new approaches for the detection of yeast and bacteria thriving in mortars
Authors: Vieira, Ricardo
González-Pérez, Marina
Pereira, António
Candeias, António
Caldeira, Ana T.
Editors: Cruz, António João
Keywords: Fluorescence In Situ Hybridization
Cultural Heritage
Microbial communities
Issue Date: 2016
Publisher: Associação Profissional de Conservadores-Restauradores de Portugal
Citation: Ricardo Vieira, Marina González-Pérez, António Pereira, António Candeias, Ana T. Caldeira.Novos desenvolvimentos para a deteção de leveduras e bactérias presentes em argamassas. Conservar Património (2016) 23, 71-77. pp. 71-77,
Abstract: The development of simple and rapid new approaches for analysing microbial communities colonising Cultural Heritage materials is pivotal for its safeguard. Fluorescence in situ hybridisation technique using ribosomal RNA directed probes (RNA-FISH) has demonstrated a great potential for this purpose. A protocol for analysing filamentous fungi in mortars has been already developed in previous studies. In this work this protocol has been adapted for detecting bacteria and yeasts. Good results have been obtained for the analysis of suspensions of isolates. In this way, the optimized protocol was applied in microsamples from synthetic mortar artificially inoculated with yeast and bacterial isolates. Promising results have been obtained for the ex situ analysis of yeast and bacteria thriving in mortar microsamples.
ISSN: 2182-9942
Type: article
Appears in Collections:HERCULES - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

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