Please use this identifier to cite or link to this item:
http://hdl.handle.net/10174/13225
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Title: | Microbial communities analysis assessed by pyrosequencing - a new approach applied to conservation state studies of mural paintings |
Authors: | Rosado, T Mirão, J Candeias, A Caldeira, A T |
Keywords: | Biodegradation Microbial diversity Pyrosequencing DGGE |
Issue Date: | 2014 |
Citation: | Rosado T, Mirão J, Candeias A and Caldeira AT (2014) Microbial communities analysis assessed by pyrosequencing - a new approach applied to conservation state studies of mural paintings, Anal Bioanal Chem, 406:887–895. |
Abstract: | The knowledge about the microbial communities
present in mural paintings is of utmost importance to develop
effective conservation and mitigation strategies. The present
paper describes a methodological approach for the detailed
characterisation of microorganisms thriving in mural paintings
by combining culture-dependent methods that allow the identification
of microorganisms capable of growing in the laboratory
conditions and to obtain high cell densities for further
studies, and culture independent methods, such as denaturing
gradient gel electrophoresis (DGGE) and pyrosequencing.
The coupled use of culture-dependent methods and DGGE
does not give enough information to investigate the diversity
and abundance of microorganisms present in wall paintings.
Pyrosequencing, a novel molecular technique, used here for
the first time in this area of research, allowed the identification
of a large number of microorganisms, confirming some already
identified by the cultivation-dependent methods such as
fungi of the genera Penicillium and Cladosporium, but also
providing a great contribution in the identification of several
genera and species, not previously identified in these artworks,
giving also a detailed overview of contaminants which was not possible with the other approaches. The results obtained
on several mural painting samples show a strong relationship
between the most deteriorated areas of the paintings
and higher microbial contamination. |
URI: | http://hdl.handle.net/10174/13225 |
Type: | article |
Appears in Collections: | CQE - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
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