DSpace Collection:http://hdl.handle.net/10174/1692024-03-29T07:47:10Z2024-03-29T07:47:10ZCompounds produced by plants and bacteria for the control of the plant- parasitic nematode Pratylenchus penetransBarbosa, PedroFaria, Jorge M. S.Figueiredo, Ana CristinaMota, ManuelVicente, Cláudia S. L.http://hdl.handle.net/10174/361982024-01-23T11:15:41Z2023-09-30T23:00:00ZTitle: Compounds produced by plants and bacteria for the control of the plant- parasitic nematode Pratylenchus penetrans
Authors: Barbosa, Pedro; Faria, Jorge M. S.; Figueiredo, Ana Cristina; Mota, Manuel; Vicente, Cláudia S. L.
Abstract: Potato (Solanum tuberosum) is one of the world’s most common agricultural crop for human consumption and considered an excellent source of essential nutrients and vitamins. The productivity of this crop is threatened by several plant-parasitic nematodes, among which the root-lesion nematode Pratylenchus penetrans is considered to have a significant impact. This organism is extremely difficult to eradicate and control procedures are expensive and hazardous to the environment and human health. Natural compounds have gained an increasingly importance as chemical substitutes; however, their effect towards plants is largely unknown. Our main goal is to identify naturally-produced compounds able to control P. penetrans and simultaneously non-harm plant host. The research plan includes: (1) in vitro bioassays of plant and bacterial compounds for biological control of P. penetrans; (2) greenhouse trials to test the selected compounds in potato cultures under nematode presence with evaluation of plant physiological response and volatile profile; and (3) preliminary studies in nematode transcriptomic changes and molecular pathways during the response to the nematicidal compounds. Results will be an add-value to the continuous investigation on environmental-friendly substances for the suppression of plant parasitic nematodes.2023-09-30T23:00:00ZElucidation of the nematicidal mode of action of 3-octanol on the root-lesion nematode Pratylenchus penetransBarbosa, PedroCosta, MarinaEspada, MargaridaFigueiredo, Ana CristinaMota, ManuelVicente, Cláudia S. L.http://hdl.handle.net/10174/361962024-01-23T11:15:29Z2023-08-31T23:00:00ZTitle: Elucidation of the nematicidal mode of action of 3-octanol on the root-lesion nematode Pratylenchus penetrans
Authors: Barbosa, Pedro; Costa, Marina; Espada, Margarida; Figueiredo, Ana Cristina; Mota, Manuel; Vicente, Cláudia S. L.
Abstract: The root-lesion nematode (RLN), Pratylenchus penetrans, is one of the most severe plant parasitic nematodes (PPN), responsible for productivity losses in a significant number of plant hosts. The chemical control of these PPNs relies on fumigants and non-fumigants compounds, which offer a systemic protection. Such control methods are costly and hazardous to the environment and to humans. Compounds naturally produced by plants may play an important role in nematode control. The aim of this study is to analyze the molecular response of P. penetrans to the nematicidal agent 3-octanol (C8H18O) using a De novo transcriptome assembly approach. Previous work showed that 3-octanol is a strong nematicide of P. penetrans causing 100% mortality in standard direct contact for 24h. The mode of action of this nematicide was studied at sub-lethal exposure for 30 min. Overall, the aliphatic alcohol 3-octanol induced the activation of detoxification mechanisms of P. penetrans with up-regulation of metabolic pathways related with metabolism of xenobiotics by CP450, steroid hormone synthesis, retinol metabolism, drug metabolism among others, while affecting ascorbate and aldarate metabolism, starch/sucrose metabolism and several signaling pathways related with cellular immune response. Understanding the defense mechanisms of P. penetrans against natural nematicides can lead to development of tailor- made control solutions.2023-08-31T23:00:00ZA molecular and spectrometric approach for tracing the origin of ancient ivoryCosta, MarinaVicente, Cláudia S. L.Espada, MargaridaFaria, JorgeTeixeira, JorgeBettencourt, JoséTeixeira, Dorahttp://hdl.handle.net/10174/361852024-01-23T11:14:31Z2023-07-31T23:00:00ZTitle: A molecular and spectrometric approach for tracing the origin of ancient ivory
Authors: Costa, Marina; Vicente, Cláudia S. L.; Espada, Margarida; Faria, Jorge; Teixeira, Jorge; Bettencourt, José; Teixeira, Dora
Abstract: The remains recovered from ancient shipwrecks are a unique cultural, historical and archaeological heritage. The Horta Bay shipwreck (site BH-001), located at Faial Island, Azores archipelago, Portugal, was partially excavated between 2008 and 2012 and became of particular interest to scientists due to the quantity and diversity of artefacts recovered, with special emphasis to a vast elephant tusks collection of unknown origin. Named as “Ivory‘s Shipwreck,” a preliminary study of the artefacts suggests that it was a merchant ship operating in the Atlantic in the early eight- eenth century, with possible English origin. This assumption needs to be supported by a full study of these rare items, resorting to a modern multi-analytical approach.
The analysis of artefacts through advanced analytical chemistry and molecular biology techniques may provide in- formation about the geographical origin of the ship, the route, its cargo, and a better understanding of the strategic importance of the Port of Horta in the 17th and 18th centuries.
The artefacts recovered from the BH-001 site cover a wide variety of materials and an extraordinary collection of about one hundred ivory elephant tusks. The material analysis of these samples can provide information about the Asian or African origin of the elephants. Although the African source of the BH-001 ivory cargo is the most probable, analytical confirmation would provide higher reliability.
In this work, we present the preliminary results obtained from the BH-001 archaeological site, using DNA-based methods for species identification (mitochondrial DNA barcoding) and spectroscopic methods, like Fourier trans- form infrared spectroscopy (FTIR) and X-ray Fluorescence (XRF). Important information was obtained for elephant species identification by optimizing DNA extraction methods, however, data confirmation must be performed using a larger number of samples from the “Ivory‘s Shipwreck” cargo and, if possible, samples obtained from other wrecks of the same chronological period.2023-07-31T23:00:00ZAvaliação socioeconómica e cultural da pesca do salmão-do-atlântico em PortugalRaposo, I.Silva, S.Alexandre, C.M.Stratoudakis, Y.Belo, A.F.Mateus, C.S.Lança, M.J.Almeida, P.R.http://hdl.handle.net/10174/361492024-01-18T10:53:13Z2023-11-18T00:00:00ZTitle: Avaliação socioeconómica e cultural da pesca do salmão-do-atlântico em Portugal
Authors: Raposo, I.; Silva, S.; Alexandre, C.M.; Stratoudakis, Y.; Belo, A.F.; Mateus, C.S.; Lança, M.J.; Almeida, P.R.
Abstract: O salmão-do-atlântico (Salmo salar) é uma espécie anádroma emblemática do
Hemisfério Norte e autóctone em Portugal. Segundo a IUCN o seu estatuto de
conservação global é Pouco Preocupante (LC), mas em Portugal encontra-se
classificado como Criticamente em Perigo (CR), sendo provavelmente a população
desta espécie mais ameaçada à escala global. A sua abundância na Europa diminuiu
em toda a área nativa, fruto de várias pressões de índole antropogénica (e.g.,
construção de barragens, poluição, pesca) ou natural (e.g., alterações climáticas). As
populações do Sul da Europa, mais especificamente da Península Ibérica que
representa o limite sul de distribuição global da espécie, são as que têm sofrido o
maior declínioao mesmo tempo que são as áreas com maior escassez de
informações.
Torna-se, por este motivo, urgente a implementação de medidas e estratégias
de gestão e conservação, que permitam manter ou mesmo promover os efetivos
populacionais desta espécie e impedir o seu desaparecimento destas áreas. Para tal,
é imperativo aumentar o conhecimento regional e local sobre a espécie, não apenas
em termos da sua ecologia, mas também sobre as atividades que lhe são dirigidas,
como é o caso da pesca.2023-11-18T00:00:00Z