Please use this identifier to cite or link to this item:
http://hdl.handle.net/10174/27544
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Title: | Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans |
Authors: | Vicente, Cláudia Mota, Manuel |
Keywords: | Pratylenchus penetrans Pectin methyl esterase |
Issue Date: | 22-Feb-2019 |
Publisher: | PLoS |
Citation: | Vicente CSL, Nemchinov LG, Mota M,
Eisenback JD, Kamo K, Vieira P (2019)
Identification and characterization of the first pectin
methylesterase gene discovered in the root lesion
nematode Pratylenchus penetrans. PLoS ONE
14(2): e0212540. https://doi.org/10.1371/journal.
pone.0212540 |
Abstract: | Similar to other plant-parasitic nematodes, root lesion nematodes possess an array of
enzymes that are involved in the degradation of the plant cell wall. Here we report the identification
of a gene encoding a cell wall-degrading enzyme, pectin methylesterase PME (EC
3.1.1.11), in the root lesion nematode Pratylenchus penetrans. Both genomic and coding
sequences of the gene were cloned for this species, that included the presence of four
introns which eliminated a possible contamination from bacteria. Expression of the Pp-pme
gene was localized in the esophageal glands of P. penetrans as determined by in situ
hybridization. Temporal expression of Pp-pme in planta was validated at early time points of
infection. The possible function and activity of the gene were assessed by transient expression
of Pp-pme in plants of Nicotiana benthamiana plants via a Potato virus X-based vector.
To our knowledge, this is the first report on identification and characterization of a PME
gene within the phylum Nematoda. |
URI: | https://doi.org/10.1371/journal. pone.0212540 http://hdl.handle.net/10174/27544 |
Type: | article |
Appears in Collections: | BIO - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
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