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|Title: ||Pinewood nematode-associated bacteria contribute to oxidative stress resistance of Bursaphelenchus xylophilus|
|Authors: ||Vicente, Claudia|
|Keywords: ||Bursaphelenchus xylophilus|
|Issue Date: ||2013|
|Abstract: ||Background: Pine wilt disease (PWD) caused by the pinewood nematode Bursaphelenchus xylophilus is one of the
most serious forest diseases in the world. The role of B. xylophilus-associated bacteria in PWD and their interaction
with the nematode, have recently been under substantial investigation. Several studies report a potential
contribution of the bacteria for the PWD development, either as a helper to enhance the pathogenicity of the
nematode or as a pathogenic agent expressing interesting traits related to lifestyle host-adaptation.
Results: We investigated the nematode-bacteria interaction under a severe oxidative stress (OS) condition using a
pro-oxidant hydrogen peroxide and explored the adhesion ability of these bacteria to the cuticle surface of the
nematodes. Our results clearly demonstrated a beneficial effect of the Serratia spp. (isolates LCN-4, LCN-16 and
PWN-146) to B. xylophilus under the OS condition. Serratia spp. was found to be extremely OS-resistant, and promote
survival of B. xylophilus and down-regulate two B. xylophilus catalase genes (Bxy-ctl-1 and Bxy-ctl-2). In addition, we show
that the virulent isolate (Ka4) of B. xylophilus survives better than the avirulent (C14-5) isolate under the OS condition.
The bacterial effect was transverse for both B. xylophilus isolates. We could not observe a strong and specific adhesion
of these bacteria on the B. xylophilus cuticle surface.
Conclusions: We report, for the first time, that B. xylophilus associated bacteria may assist the nematode
opportunistically in the disease, and that a virulent B. xylophilus isolate displayed a higher tolerance towards the OS
conditions than an avirulent isolate.|
|Appears in Collections:||BIO - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica|
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